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KMID : 0364820220580040272
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Korean Journal of Microbiology
2022 Volume.58 No. 4 p.272 ~ p.285
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Brevundimonas fontaquae sp. nov., isolated from Dalgi carbonate spring-water
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Lee Kyoung
Son Hae-Lim
Choi Yu-Jin
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Abstract
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Brevundimonas sp. CS1T, isolated from Dalgi carbonate spring water in Cheongsong-up, Korea, is Gram-negative, aerobic, motile, and rod-shaped. Cells (0.5 μm × 1.2?2.6 μm) contain a single polar flagellum. The isolated colonies were round, viscous, and an orange color when incubated for 3 days at 28°C on nutrient agar. Furthermore, the strain (CS1T) produces a diffusible brown pigment when grown on tryptic soy broth agar. CS1T was positive for oxidase, DNase, and catalase activities, and positive for Tween 20, carboxymethyl cellulose, and starch hydrolysis. CS1T was further characterized using API 20 NE, API 50CH, and API ZYM kits. The major fatty acids included C18:1 ω7c/C18:1 ω6c (47.31%) and C16:0 (17.61%). Non-phosphorylated glycolipids were the dominant polar lipids. Coenzyme Q-10 was predominantly detected as a quinone in this strain. The CS1T genome was sequenced using Illumina and Oxford nanopore sequencing platform. The genome size was 3.3 Mb with a G + C content of 66.1 mol%. No plasmids, CRISPR array genes, or complete prophages were detected. Interestingly, the genome encodes P-type conjugative transfer proteins. Average nucleotide identity (ANI) value between CS1T and the most closely related type strain, B. vesicularis NBRC 12165T, was 94.27%. Comparison of CS1T and strains to closely related type species based on polyphasic taxonomic analysis, ecology, and genome-based phylogeny indicated strain CS1T is a novel species. Thus, we propose the name Brevundimonas fontaquae for CS1T, whose strain type is CS1T (= KCTC 82559T = JCM 34943T).
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KEYWORD
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Brevundimonas fontaquae sp. nov., Brevundimonas vesicularis, carbonate spring water, Cheongsong-up, Dalgi
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